TSH levels, overweight, BMI, and skin expression levels of DCT and CCBL2 genes are related to vitiligo treatment response with narrow band UVB phototherapy


Jorge Ocampo-Candiani 1, Mauricio Salinas-Santander 2, Rocio Ortiz-López 3, Eduardo de la Rosa-Moreno 4, Victor Trevino 3, Juan José Vilata-Corell 5, Osvaldo Vázquez-Martínez 1, Jorge Ocampo-Garza 1, Celia Sánchez-Domínguez 6, José Luis Alfonso-Sánchez 7, Alejandra Villarreal-Martínez 1, Verónica Garza-Rodríguez 1

1Facultad de Medicina, Servicio de Dermatología, Universidad Autónoma de Nuevo León, Hospital Universitario Dr. José Eleuterio González, Monterrey, Nuevo León, Mexico.
2Departamento de Investigación, Facultad de Medicina Unidad Saltillo, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
3Escuela de Medicina, Tecnológico de Monterrey, Monterrey, Mexico.
4Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
5Departamento de Dermatología, Universitat de València, Hospital General Universitario de Valencia, Valencia, Spain.
6Departamento de Bioquímica y Medicina Molecular, Universidad Autónoma de Nuevo León, Facultad de Medicina, Monterrey, Nuevo León, Mexico.
7Departamento de Medicina Preventiva y Salud Pública, Universitat de València, Hospital General Universitario de Valencia, Valencia, Spain.

*Email: jocampo2000@yahoo.com.mx

Vitiligo is a chronic skin disease characterized by a lack of pigmentation in the skin of affected patients (Boniface, Seneschal, Picardo, & Taieb, 2017). The study of the functional status of the skin could be helpful in predicting the response to phototherapy treatment. To date, no studies about clinical, biochemical, and gene expression profiles related to response to treatment are available, particularly with narrowband ultraviolet B light phototherapy (nb-UVB), the first choice treatment in vitiligo (Majid, 2010). Therefore, and to identify clinical, biochemical, and skin gene expression patterns with prognostic utility, we conducted a study with 45 subjects with vitiligo vulgaris (VV): 23 patients with active VV (AVV, 10 male and 13 female) and 22 patients with stable VV (SVV, 14 male and 8 female). Two skin biopsies (from vitiligo lesions and TABLE 1 Family history and early onset of vitiligo pigmented areas) of 4 mm in diameter were obtained before the administration of 48 nb-UVB phototherapy sessions, performed two times a week for a period of 6 months with an initial dose of 150 mJ/cm, and increments of 50 mJ/cm 2 every third session until the minimum dose of asymptomatic erythema was achieved. Each biopsy was used to isolate total RNA and to perform expression analysis with TruSeq Targeted RNA Expression analysis (RNA-Seq, Illumina, Inc., San Diego, CA) (Ocampo-Candiani et al., 2018), targeting 29 genes involved in skin pigmentation, apoptosis, cell survival, and signal transduction mechanisms.

1 TSH-levels-overweight-BMI-Ocampo-Can-l-2019-Dermatol_Therapy