Thymidylate synthase polymorphism in Mexican patients with colon cancer treated with 5-fluorouracil

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Clara Patricia Rios-Ibarra1,2*, Claudia Janeth Rodriguez-Silva3, Yamil Alonso Lopez-Chuken3, Rocio Ortiz-Lopez1,4, Elisa Fernandez-Castillo2, Claudia Beatriz Del Toro Runzer2, Vicente Paul Armenta-Perez2, Juan Pablo Flores-Gutierrez5, Arturo Quintanilla-Guzman1, Mauricio Salinas-Santander7, Juan Francisco Gonzalez-Guerrero3, Francisco Bosques-Padilla6, Arturo Santos2, Herminia Guadalupe Martinez-Rodriguez1*

1 Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Mexico (UANL);
2 Department of Bioengineering, Tecnologico de Monterrey, campus Guadalajara;
3 University Cancer Center and
4 Center for Research and Development in the Health Sciences, Autonomous University of Nuevo Leon, Mexico;
5 Service of Anatomic Pathology and Cytopathology, School of Medicine and University Hospital “Jose Eleuterio Gonzalez”, UANL;
6 Service of Gastroenterology, School of Medicine and University Hospital “Jose Eleuterio Gonzalez”, UANL;
7 School of Medicine, Autonomous University of Coahuila, Mexico

*herminiamar@gmail.com

Purpose: We analyzed the genotype and allele frequency of variable number tandem repeats (VNTR)-thymidylate
synthase (TS) and its relationship with the disease evolution in colon cancer patients.
Methods: We selected 24 paraffin-embedded colon cancer tissue samples from Mexican patients who received a 5-fluorouracil (5-FU)-based chemotherapy regimen. Tumor tissue was digested with proteinase K and genomic DNA was isolated by the standard method with phenol-chloroform extraction. Polymerase chain reaction (PCR) was performed for TS genotyping of VNTR and the results were evaluated directly in a stained agarose gel.
Results: The allele frequency of 2 repeats (2R) was greater (0.66) than 3R (0.34) in metastatic colon cancer (x2=10.24; p=0.001); however, no difference in allelic distribution between 2R (0.54) and 3R (0.46) in non metastatic patients was observed (x2=0.640; p=0.424).
Conclusion: Our results suggest that Mexican patients with colon cancer present differences in the allelic distribution, the 2R allele being the most frequent.

2 ARTÍCULO-JBUON-AGO-2016 Thymidylate synthase C Rios


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