The matrix synthesis and anti-inflammatory effect of autologous leukocyte-poor platelet rich plasma in human cartilage explants


To determine the effects of autologous leukocyte-poor platelet-rich plasma (LPPRP) on the expression of markers involved in cartilageextracellular matrix production and inflammation in cartilage explants bearing osteoarthritis. Cartilage explants and LP-PRP were obtained from 10 patients who underwent total knee arthroplasty.
The explants were cultured in spinner flasks for 28 days in the presence of interleukin (IL)- 113 and/or LP-PRP.
The gene expression of catabolic (MMP13, ADAMTS5, and Hifi) and anabolic factors (COL2A1, ACAN, and SOX9) was quantified.

2018 The matrix synthesis and anti-inflammatory