Recovery and Analysis of the Biological Activity of Lycopene Produced by Rhodotorula glutinis P4M422


Ayerim Hernandez-Almanza1, Jae Kweon Park2, Nallely E. Carlos-Interial1, Jose G. Fuentes-Aviles1 , Cristobal N. Aguilar1∗

1 School of Chemistry. Autonomous University of Coahuila. Saltillo, 26280, Coahuila, Mexico
2 Department of Life Sciences, Gachon University. Seongnam-si, 461-701, Gyeonggi-do, South Korea


Lycopene is a carotenoid intermediate of the β-carotene biosynthetic pathway. It is obtained from vegetable source and chemical synthesis. It has roused considerable interest due to its beneficial effect on human health, e.g. cancer and tumor prevention, cardiovascular protection and antioxidant activities. Due the increasing interest of this bioactive molecule, it is necessary to develop alternative methodologies to produce it in higher amounts, for example, the microbial production. In the present study, the lycopene production by the yeast Rhodotorula glutinis P4M422 was evaluated. R. glutinis was inoculated in YM broth at 30 ◦C and 130 rpm. Incubation time optimization and imidazole effect in the specific accumulation of lycopene were evaluated. On the other hand, the antioxidant and thermal stability of lycopene capsules (GNC Mexico) were evaluated. The effect of imidazole at different concentrations (0, 1.5, 3, 6 and 12 mM) was studied, however, the results show that the highest biomass production and the major carotenoids concentration was obtained in absence of imidazole. Also, different organic solvents were evaluated in carotenoids extraction; we found that the use of methanol allowed the highest lycopene extraction (0.01 g/L). Carotenoids are a pigments with antioxidant activity, in the present study, ABTS, FRAP and DPPH(78.9, 71.19 and 44.77 mg/L Trolox equivalent, respectively) assays showed this property.