Facultad de Ciencias Químicas
Ing. Laura María Solís Salas
Abstract: Citrus sinensis L. is cultivated in Mexico as food; the epicarp contains a variety of chemical compounds with biological properties with health benefits. The objective of this work was extract, purify and identify the compounds obtained residue epicarp of Citrus sinensis L. and evaluate its biological activity, on A. salina and cell cultures. The extracts were obtained with organic solvents (hexane, acetone and methanol), extract with increased activity on A. salina were fractionated by liquid chromatography column under vacuum (VLC), inverted column chromatography (ICC) and thin layer chromatography (TLC), fractionations were bio-guided with A. saline nauplii and the cell line murine melanoma (B16F10), then cytotoxicity on murine lymphoma cell line (L5178Y) and peripheral blood mononuclear cells was evaluated. Bioactive fractions isolated by VLC and ICC were characterized by qualitative phytochemical analysis. The extract and the bioactive fractions isolated by TLC were characterized by spectroscopic and spectrophotometric methods. The hexane extract showed increased activity on A. salina that the methanol and acetone. The bioactive fractions isolated by TLC were found sugars, total phenols phenolic compounds, coumarins and saponins. Also in the bioactive fractions, antioxidant effect was observed by testing DPPH and FRAP and higher antioxidant effect was found in the TLC3-2 fraction. The murine melanoma cell line (B16F10) allowed selection of bioactive fractions. The fractions with higher cytotoxic effect on the murine melanoma cell line (B16F10) were TLC23, TLC32 and TLC36 fractions also showed selectivity in cytotoxicity on murine lymphoma cell line (L5178Y) and cells peripheral blood.