Facultad de Ciencias Químicas
Ing. María Elena Velázquez Arellano
Abstract: The production of pigments by different strains of Monascus has been carried out in solid fermentation and submerged fermentation in various agroindustrial residues, which have multiple applications in the food, cosmetic, pharmaceutical and textile industries. In this research the use of Aloe vera leaves as substrate for the production of red, yellow and orange pigments by Monascus purpureus CECT2955 in liquid and solid fermentation was evaluated. Aloe vera leaves were evaluated for viability under a pre-treatment (acid hydrolysis) as an alternative substrate. Different concentrations of H2SO4 (0.5, 1.0 and 1.5% v / v) were evaluated at different times (30, 60 and 90 min) as hydrolysis treatments in two types of enrichment media addition of salts and without salts. According to the results, it was observed that the highest production of red and yellow pigments (8.19 ± 0.20 UA495nm and 6.32 ± 0.84 UA410nm respectively) were obtained using hydrolyzate (hydrolysis conditions: 0.5% H2SO4 for 90 min) with the addition of salts. Subsequently, the effect of the inoculum type (spores and mycelium) on the production of pigments by Monascus purpureus CECT2955 using Aloe vera hydrolysates was evaluated and the conditions of preparation of the previously selected inoculum were optimized to maximize pigment production by Monascus purpureus CECT2955 in submerged culture using Aloe vera hydrolysates as substrate.