Producción de licopeno por Rhodotorula glutinis YB-252 en un sistema de cultivo en estado sólido


Facultad de Ciencias Químicas

QFB Ayerim Yedid Hernández Almanza

Abstract: Carotenoids represent a group of valuable molecules whit specific applications as colorants, food supplements and nutraceuticals. Despite the availability of an variety of natural and synthetic carotenoids, only few of them have been exploited commercially, including beta-carotene, astaxanthin and lycopene. Lycopene, an intermediate in the biosynthetic pathway of beta-carotene and it is the most potent antioxidant. Commercial production is manly based on chemical synthesis and extraction from natural sources, such as tomatoes. Nonetheless, due to the increasing importance of this molecule, alternative methodologies to produce higher quantities of carotenoids have been developed. The aim of this work was to produce biotechonologically lycopene through a system of solid-state fermentation for Rhodotorula glutinis YB-252. An evaluation of different solid matrices (polyurethane foam, sugarcane bagasse and rice bran) and bioreactores (tray and column) for pigment production was evaluated. Polyurethane foam and column, favored the production. Plackett-Burman design was used to evaluate the effect of pH, moisture, carbon source, inoculum concentration, time and aeration and the concentration of regulator.

Thesis Ayerim Yedid Hernandez Almanza