Producción de la enzima tanin-acil hidrolasa por fermentación en estado sólido usando residuos de té verde y pulpa de café como soporte


Facultad de Ciencias Químicas

QFB. Erick Maurilio Peña Lucio

Abstract: The present project focuses on the production of the tannin-acyl hydrolase enzyme using green tea residues and coffee pulp as support for solid-state fermentation, the overall objective was the optimization of the fermentation process for obtaining tanasses using endemic strains from ¨Western Ghats¨ in India and ¨Cuatrociénegas¨, Coahuila, México. The “Western Ghats” are a particular ecosystem with plants rich in tannins and very high temperatures during the day and very low at night, due to this, the strains belonging to these environments have a great capacity for adaptation. Cuatrociénegas is a unique area because of the ecosystems found there, which are similar to those of millions of years ago. The use of agro-industrial residues of green tea and coffee pulp is an alternative for obtaining metabolites of interest Solid-state fermentation, it which has been defined as a bioprocess that is performed in the absence of free water was used to Obtaining hydrolytic enzymes (tannase) by agro-industrial wastes (coffee pulp and green tea). Tannin-acyl hydrolase (TAH) commonly known as tannase (EC. is responsible for the degradation of tannins, this enzyme can hydrolyze the bonds present in the gallotannins, ellagitannins and complex tannins to produce gallic acid and glucose.

Thesis Erick Maurilio Peña Lucio