Microbial diversity and biochemical profile of aguamiel collected from Agave salmiana and A. atrovirens during different seasons of year


M. Isabel Enrıquez-Salazar1, Fabiola Veana2, Cristobal N. Aguilar1, Iliana M. De la Garza-Rodrıguez3, Mercedes G. Lopez4, Olga M. Rutiaga-Quiñones5, Jesus A. Morlett-Chvez1, Raul Rodrıuez-Herrera*1

1 Food Research Department, School of Chemistry, Universidad Autonoma de Coahuila, 25280 Saltillo, Coahuila, Mexico
2 Present Address: Instituto Potosino de Investigacion Cientıfica y Tecnologica A.C., 78216 San Luis Potosı, Mexico
3 Analytical Chemistry Department, School of Chemistry, Universidad Auto´noma de Coahuila, 25280 Saltillo, Coahuila, Mexico
4 Biotechnology and Biochemistry Department, Centro de Investigacion y de Estudios Avanzados del IPN, Unidad Irapuato, 36821 Irapuato, Guanajuato, Mexico
5 Instituto Tecnolo´gico de Durango, 34080 Durango, Mexico


Aguamiel is a beverage produced by some Agave species that is consumed in its fresh or fermented form. Despite its uses and popularity, seasonal effects on its microbial and chemical profiles are unknown. In this
study, using aguamiel collected from A. salmiana and A. atrovirens during different seasons, we identified microorganisms by sequencing the 16S and 18S rDNA genes and determined their chemical profiles. In total, 49
microbial strains were identified (38 bacteria and 11 yeasts). The highest richness and biodiversity were
observed during winter and summer. Different lactic acid bacteria and yeast genera with potential industrial applications were identified, such as Acetobacter, Lactobacillus, Leuconostoc, and Clavispora. The analysis of the chemical profiles indicated the presence of maltooligosaccharides and fructooligosaccharides, which are associated with human health improvements, during spring in Agave aguamiel. Aguamiel can be used in the food industry due to its microbiological and chemical profiles.

18 ART 2017 copia