Inulin Potential for Enzymatic Obtaining of Prebiotic Oligosaccharides


Adriana C. Flores1, Jesús A. Morlett1 & Raúl Rodríguez*1

1Food Research Department, School of Chemistry, Universidad Autonoma de Coahuila. Boulevard Venustiano Carranza and José Cárdenas s/n. Col. Republica Oriente. Zip Code 25280, Saltillo, Coahuila, México.


Oligosaccharides have been marketed since the 1980s as low calories agents and recently have gained interest in the pharmaceutical and food industry as functional sweeteners and prebiotic enriching population of Bifidobacteria. Currently, they have an approximated value of $ 200 per kg and recently, inulin has been proposed as a feedstock for production of oligosaccharides through selective hydrolysis by action of endoinulinase. High optimum temperature (60 °C) and thermostability are two important criteria which determine suitability of this enzyme for industrial applications as well as enzyme cost, a major limiting factor. Significant reduction in cost can be achieved by employing low-value and abundant inulin rich plants as Jerusalem artichoke, dahlia, yacon, garlic and onion, among others. In general, the early-harvested tubers of these plants contain a greater amount of highly polymerized sugar fractions, which offer more industrial value than late-harvested tubers or those after storage. Also, development of recombinant microorganisms could be useful to reduce the cost of enzyme technology for large scale production of oligosaccharides. In the case of fungal inulinases, several studies of cloning and modification have been made to achieve greater efficiency. The present article reviews inulin from vegetable sources as feedstock for oligosaccharides production through the action of inulinases, the impact of polymerization degree of inulin and its availability and some strategies to increase oligosaccharides production.

12 ART 2015