Inhibition of miR31 and miR92a as Oncological Biomarkers in RKO Colon Cancer Cells Treated with Kaempferol-3-O-Glycoside Isolated from Black Bean


Janet Alejandra Gutierrez-Uribe,1 Mauricio Salinas-Santander,2 Delia Serna-Guerrero,3 Sergio Roman Othon Serna-Saldivar,3 Ana Maria Rivas-Estilla,4 and Clara Patricia Rios-Ibarra5

1 Tecnologico de Monterrey, Department of Bioengineering and Science, Puebla, Mexico.
2 Research Department School of Medicine Saltillo Unit, Autonomous University of Coahuila, Coahuila, Mexico.
3 Tecnologico de Monterrey, Protein Research and Development Center, Monterrey, Mexico.
4 Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon,
Nuevo Leon, Mexico.
5 Tecnologico de Monterrey, Department of Bioengineering, Guadalajara, Mexico


MicroRNAs (miRNAs) are small molecules of 19–23 nucleotides of RNA that act as regulators of the expression of proteins in eukaryotic cells. Currently, the participation of miRNAs in the development of different types of cancer has been observed. To evaluate the inhibitory effect of kaempferol-3-O-glycoside on the expression of oncological biomarkers, miR31 and miR92a in a colon cancer cell line (RKO) were analyzed. Cells were cultured and treated with 1 mM kaempferol-3-O-glycoside isolated from black bean. Expression levels of miR31 and miR92a were evaluated by real-time PCR using TaqMan probes; in addition, two oncogenes (KRAS and c-MYC) and two tumor suppressors (AMP-activated protein kinase [AMPK] and adenomatous tumors of polyposis coli [APC]) were quantified to validate the biological effects; normalization of expression levels were carried out by 2−ΔΔCt. Results were analyzed by one-way ANOVA. The expression levels of miR31, miR92a, KRAS oncogene, and the c-MYC transcription factor were subexpressed upon 72 h post-treatment with kaempferol-3-O-glycoside compared with the control without treatment (P < .05); in contrast, the tumor suppressor genes AMPK (∼4.85, P = .005) and APC (∼2.71, P = .066) tumor suppressors genes were overexpressed. Our results showed the inhibitory effect of isolated black bean flavonoid kaempferol-3-O-glycoside on cancer biomarkers: miR31 and miR92a; based on our results, this flavonoid may have interesting nutritional, therapeutic, and/or prophylactic applications to combat colon cancer.

1 ARTICULO- Journal of Medicinal Food-jmf.2019.0059-publicado 2019