Fast acetone tissue processing of human organs provides tissue characteristics equal to conventional processing


Routine preparation of paraffin embedded tissue for histopathological diagnosis, here termed
conventional histological technique (CT), whether performed manually or using an automated
system, requires approximately 12 h. We developed earlier a rapid acetone dehydration technique
(AT) for processing biopsies of nervous tissue that meets requirements for preserving tissue
morphology and staining properties, and reduces processing time to 3.3 h. We compared the
morphology and staining properties of human organ biopsies including adrenal gland, liver,
ovary, pancreas, prostate, testis and thyroid prepared using both AT and CT.

2020 Fast acetone tissue processing of human-2