Evaluation of the gallic acid effect on the proteome of Huh7 hepatocarcinoma cells that express Hepatitis C Virus

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Abstract: In the present work the results obtained from the evaluation of the effect of gallic acid treatment (100, 200 and 300 μM) on hepatocarcinoma Huh7 cells transfected with a subgenomic replicon that express non-structural proteins of the hepatitis C virus will be discussed. As well as the effect generated in human hepatocarcinoma Huh7 parental cells. In the obtained results it was observed that gallic acid generated a 40% decrease in viral RNA expression at different treatment times (24, 48 and 72 hours). It also modulated the expression of the non-structural proteins of the virus (NS3 and NS5A), decreasing its expression by up to 60% since 24 h of treatment. In addition, gallic acid generated changes in the protein profile of Huh7 replicon cells, modifying the expression of proteins involved in various processes such as cell defense, oxidative stress, apoptosis, angiogenesis, and mitochondrial metabolism, among others. On the other hand, effects on the bioenergetic metabolism of both cell lines were observed, where the gallic acid treatment decreased ATP production, glycolysis and, in the case of Huh7 parental cells, decreased mitochondrial respiration. For this reason, based on the results obtained, gallic acid could be considered as a potential candidate as an adjuvant in the treatment of hepatitis C virus.

Thesis Mayela Govea

 


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