Facultad de Ciencias Químicas
Gerardo Espinosa Velázquez
Brown algae were used as first time in medicine since sixteenth century, as has been reported in literature in ancients Chinese Herbal Medicine books. Actually, seaweeds are commonly used with different purpose like: anti-allergy, anti-inflammatory, antithrombotic, anticoagulant, antitumoral, antiviral, antioxidant, immunnomodulatory, and among other propierties. This research work was focused on the process extraction of fucoidan – sulfated polysaccharides employing several techniques. The first part of this thesis describes how two species of brown seaweed were analyzed by proximal methods. Then, a second part of this project includes three types of extraction process (EAE – enzyme assisted extraction, MAE – microwave assisted extraction, ETACH – extraction assisted technology by cavitation hydrothermodynamic). Paralelly other part was focused in a new technique to identify fucoidan (U. pinnatifida) and agave fructans. Finally in another part, three diferents and commercial fucoidans were analyzed as antioxidant, capacity gel formation with a laccase enzyme, and their toxicity (CL50) by A. salina as a biological model was carried out.