Environmental friendly deproteinization and saccharification of industrial fungal biomass by enzymatic processing


Cecilia Perez-Cruz1, Carlos N. Cano-Gonzalez1, Jose Fuentes1, Nagamani Balagurusamy2, Carolina E. Vita3, Roque A. Hours3, Cristobal N. Aguilar1, Sebastian F. Cavalitto3, and Juan C. Contreras-Esquivel1,4*

1School of Chemistry, Universidad Autonoma de Coahuila, Saltillo City 25280, Coahuila State, Mexico

2School of Biological Science, Universidad Autonoma de Coahuila, Torreon City 27104, Coahuila State, Mexico

3Center for Research and Development of Industrial Fermentation (CINDEFI), School of Science, National University of La Plata. 47 y 115 (B1900ASH), La Plata, Buenos Aires, Argentina

4Research and Development Center, Coyote foods Biopolymer and Biotechnology Co., Saltillo City 25000, Coahuila State, Mexico.

*carlos.contreras@uadec.edu.mx; coyotefoods@hotmail.com

Aspergillus niger biomass, an industrial by-product of citric acid fermentation is an emergent source of glycoderivatives with applications in biofuel, cosmetics, feed, energy, food, medicine, and nanotechnology. In this study, the effect of purified neutral protease for deprotenization of fungal biomass studied at various levels (0, 5, 10, 20 and 40 U/100 mg of biomass) and the saccharification of fungal biomass was evaluated with amylolytic enzymes and chitosanases. The efficiency of deproteinization of fungal biomass was based on the enzyme concentration and contact time. Protease at a concentration of 20 U/100 mg of dry biomass and with a contact time of 8 h achieved 30% final deproteinization. No effect on saccharification of A. niger biomass was observed by treatment with purified amylolytic enzymes. Meanwhile, the endo- and exo-chitosanases treatment yielded 54 g of g reducing sugars (equivalent to amino sugars)/ kg of fungal biomass, which can be employed for tailor-made carbohydrate production.