EFECTO DEL QUITOSANO PARA EL BIOCONTROL DE HONGOS FITOPATOGENOS IDENTIFICADOS MOLECULARMENTE DE FRUTAS Y HORTALIZAS EN GUANAJUATO

publicaciones

Castañeda-Ramírez JC1*, Laurel-Ángeles V1 , Espinoza-Zamora J1, Salcedo-Hernández R3, LópezRamírez ME1, De la Fuente-Salcido NM2

1 Universidad Tecnológica del Suroeste de Guanajuato, Procesos Alimentarios. Carr. Valle-Huanímaro Km. 1.2, C.P. 38400, Valle de Santiago, Guanajuato, México.
2 Universidad Autónoma de Coahuila. Dpto. de Posgrado. Escuela de Ciencias Biológicas, Ciudad Universitaria, Campus Torreón, Coahuila, México.
3 Universidad de Guanajuato Campus Irapuato-Salamanca, División de Ciencias de la Vida, Dpto. Alimentos. Irapuato, Guanajuato, México.

*jccastanedar@utsoe,edu.mx

Chitosan is a deacetylated derivative of chitin, has a great antibacterial and antifungal capacity. The objective of
this work was the isolation and identification of fungi and evaluating the antifungal effect of chitosan of medium and
low molecular weight. For identifying fungi were isolated from fruits and vegetables contaminated analyzing macro
and microscopic characteristics and subsequent amplification and sequencing of ITS; for evaluating antifungal
effect strawberry, baby carrots and cherry tomato was used, which were disinfected with 70% ethanol (v / v) for 1
min, then with a 1% (v / v) sodium hypochlorite and rinse with distilled water; finally coated with chitosan solutions
of medium and low molecular weight (0.5, 1, 1.5, 2.0, 2.5 and 3.0% w / v) and inoculate with spores of Fusarium
oxysporum, Rhizopus oryzae, Rhizopus stolonifer, Collectotrichum gloesporoides, leaving temperature
environment 5 to 7 days. It was possible to isolate and identify the fungi of the genera Rhizopus and Fusarium. The
low molecular weight chitosan to 2.5 and 3.0% (w / v) antifungal effect showed the greatest protection for
postharvest strawberry, tomato and carrot against the fungi tested, for 6 days at room temperature, considered an
ideal agent for biocontrol phytopathogenic.

Norma5-2016


COMPARTE