Desarrollo de un bioproceso para la producción de jasmonatos a partir de Botryodiplodia theobromae


Abstract: The need to have environmentally friendly products, which can contribute to the protection of plants, the increase of agricultural yields, the formulation of food, medicines and cosmetic products is a demand of today’s society. One of these products are the jasmonates to which for many years multiple benefits have been found in these sectors. However, new processes with higher productivity and lower costs that allow the extensive use of jasmonates are required. The microbial pathway from the fungus Botryodiplodia theobromae and through a fermentation process in solid medium offers the opportunity to develop more productive processes and the use of industrial waste, which in many cases constitute environmental problems. Taking this as a starting point, the aim of this study is to design a bench-scale bioreactor that allows to evaluate the kinetic systems and heat and mass transport of a process of jasmonates production by Botryodiplodia theobromae in solid state fermentation using an industrial waste. For this, a process of recovery of jasmonates was developed initially, for its use in fermented solids. Methanol, acetone, and ethyl acetate were evaluated as solvents for the recovery of jasmonic acid (JA). For the study, orange residuals were used as a matrix to which standard JA was added and the humidity was adjusted to 70%. The parameters studied were the extraction time, the liquid-solid ratio, the stirring speed, the temperature and the number of extractions. As a response variable, the JA was quantified by high performance liquid chromatography (HPLC). The most favorable conditions were obtained using ethyl acetate, with a contact time of 30 min, liquid-solid ratio 5 mL / g, stirring speed 120 rpm, temperature 30 ° C and 3 extractions. These conditions were validated obtaining a yield in the recovery process of 97%.

Thesis PhD Gerardo Rodríguez Cutiño