Consolidated bioprocessing of hydrogen production from agave biomass by Clostridium acetobutylicum and bovine ruminal fluid


Claudia M. Lopez-Badillo

The biological production of H2 represents a renewable and eco-friendly energy alternative
compared to fossil fuels. However, its production from lignocellulose involves the use of
expensive enzymatic complexes. In the present work, the production of H2 from pretreated
agave biomass was evaluated by means of a Consolidated Bioprocess (CBP). This strategy
was carried through the interaction of cellulose-degrading microorganisms obtained from
bovine ruminal fluid (BRF) capable of enhancing H2 production by Clostridium acetobutylicum.
The results obtained show the capacity of BRF to hydrolyze the acid pretreated agave,
improving the production of H2 in the experiments where the inoculum of Clostridium was greater. According to the results, production of H2 is significantly affected by the increase of
the solids loading, obtaining a maximum H2 production at a 10% of solids loading, pH 5.5
and 35 C, representing a yield of 150 L of H2 per Kg of biomass in 264 h.
© 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

Artículo-CMLB 2 2020