José Carlos Meneses-Reyes1, Guadalupe Hernández-Eugenio1, David H. Huber2,3, Nagamani Balagurusamy4, Teodoro Espinosa-Solares1*
1Posgrado en Ingeniería Agrícola y Uso Integral del Agua, Universidad Autónoma Chapingo, Chapingo, Estado de México 56230, Mexico
1Gus R. Douglass Institute, West Virginia State University, Institute, WV 25112-1000, USA
3Department of Biology, West Virginia State University, Institute, WV 25112-1000, USA
4Laboratorio de Biorremediación, Escuela de Ciencias Biológicas, Universidad Autónoma de Coahuila, Torreón 27000, Coahuila, Mexico
The objective of this work was to evaluate the technical feasibility of using both oil-extracted microalgae (M) and glycerol (G) in co-digestion with chicken litter (CL), thereby improving biochemical methane potential (BMP). Different feedstock ratios of M (0–30%), G (0–3%) and CL (67–100%) were investigated to determine the best co-digestion condition under mesophilic conditions. According to the modified Gompertz model, the best BMP (131.1 mL CH4 g-1 VS fed) was obtained with the triple co-digestion (M:G:CL) in a proportion of 30:3:67. This yielded a methane production rate of 3.3 mL CH4 g-1 VS fed d-1 and a lag time (k) of 17.4 d. This treatment reduced chemical oxygen demand (COD) by 91.02% and increased the methane yield 15.8% with respect to the CL control.