ACE, APOA5, and MTP Gene Polymorphisms Analysis in Relation to Triglyceride and Insulin Levels in Pediatric Patients


Lilia Carranza-Gonzalez,1 Rafael B.R. Leon-Cachon,2 Marıa Antonia Gonzalez-Zavala,1 Clara Rıos-Ibarra,3 Jesus Morlett-Chavez,4 Celia Sanchez-Domınguez,5 Ana Cepeda-Nieto,6 and Mauricio Salinas-Santander6

1 Laboratorio de Analisis Clınico, Facultad de Ciencias Quımicas, Saltillo, Universidad Autonoma de Coahuila, Coahuila, Mexico
2 Departamento de Ciencias Basicas, Division de Ciencias de la Salud, Universidad de Monterrey, San Pedro Garza Garcıa, Nuevo Leon, Mexico
3 Departamento de Bioingenierıa, Tecnologico de Monterrey, Campus Guadalajara, Guadalajara, Mexico
4 Departamento de Investigacion Alimentaria, Escuela de Quımica Saltillo, Universidad Autonoma de Coahuila, Coahuila, Mexico
5 Departamento de Bioquımica y Medicina Molecular, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Nuevo Leon, Mexico
6 Departamento de Investigacion, Facultad de Medicina de la Unidad Saltillo, Universidad Autonoma de Coahuila, Saltillo, Coahuila, Mexico


Background and Aims. Obesity is a complex, chronic, and multifactorial disease that has become a major, and worldwide, public health problem contributing to an increased number of pathologies, including type 2 diabetes, cardiovascular disease, hyperlipidemia, and metabolic syndrome, thus suggesting a commolon origin. A diet high in sugar and fats coupled with a sedentary lifestyle has a major role in the development of obesity. However, the genetic background has also been associated with body fat accumulation. The aim of this study was to assess the effect ofACE-rs4646994, APOA5-rs662799, and MTP-rs1800591 gene polymorphisms on clinical and biochemical parameters and to evaluate the association with body phenotypes in children and adolescent population of Saltillo, Coahuila, Mexico. Methods. Anthropometric, clinical, biochemical parameters and BMI were obtained from 405 children and adolescents. The BMI was used to determine the body phenotype. The rs4646994 gene polymorphism was determined by PCR, whereas rs662799 and rs1800591 were determined by PCR-RFLP. The obtained results were analyzed to determine their association of these single nucleotide polymorphisms with body phenotype
and biochemical parameters.
Results. TT genotype for APOA5-rs662799 was associated with increased levels of HDL-C in the analyzed population ( p !0.05). The ACErs4646994gene polymorphism is associated with high Insulin levels, HOMAIR index, and triglyceride levels, mainly when presenting a I/I genotype ( p !0.05).
Conclusion. The polymorphic allele of the ACE gene is capable of modulating triglyceride levels, insulin levels and HOMA-IR index in the evaluated population; it must be highlighted that this has not been reported in other studied populations elsewhere.

22 ART 2018