A quick and effective method for evaluating substrate-enzyme systems in the enzymatic hydrolysis of lignocellulosic biomass


Javier Ulises Hernández-Beltrán & Karla Cervantes-Quintero & Héctor Hernández-Escoto*

Departamento de Ingeniería Química, Universidad de Guanajuato,
Noria Alta s/n, 36050 Guanajuato, GTO, Mexico.


This work describes a microreaction technique to carry out hydrolysis of certain substrate by certain enzyme under several conditions, and reports its usefulness to determine effective process conditions and to compare performances between different systems substrate-enzyme, in a quick and economic way. The microreaction system consists of microtubes, as microreactors, that are placed into a thermomixer, allowing a set of 24 experiments at a certain constant temperature but with different pH and substrate-enzyme ratios. The outcomes of the numerous experiments are processed via response surface methodology and means analysis. In order to illustrate the technique effectiveness, it is discussed the comparison of two commercial enzyme complexes, and two delignification procedures of wheat straw.