Fermentation of Dietetic Fiber from Green Bean and Prickly Pear Shell by Pure and Mixture Culture of Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B

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Y. N. Mora‑Cura1 · N. P. Meléndez‑Rentería1 · M. Delgado‑García1,2 · J. C. Contreras‑Esquivel1 · J. A. Morlett‑Chávez3 · C. N. Aguilar1 · R. Rodríguez‑Herrera*1

1 Departamento de Investigación en Alimentos, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila,
Boulevard Venustiano Carranza and José Cárdenas s/n, República Oriente, 25280 Saltillo, Coahuila, Mexico
2 Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270 Guadalajara, Jalisco, Mexico
3 Laboratorio de Diagnóstico Molecular y Clínico, Facultad de ciencias Químicas, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza and José Cárdenas s/n, República Oriente, 25280 Saltillo, Coahuila, Mexico

*raul.rodriguez@uadec.edu.mx

The aim of this study was to evaluate the fermentation of dietary fiber from green bean (Phaseolus
vulgaris) and prickly pear shell (Opuntia ficus-indica) by Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B growing as mono-culture and co-culture, the fermentation products, and proteins expressed during this process. The analysis of the fermentation profile showed a major growth of bacteria in the culture media of each
dietary fiber supplemented with glucose, and particularly B. bifidum 450B at 48 h showed the highest growth. In the
case of the co-culture, the growth was lower indicating the possible negative interaction between L. acidophilus LA-5
and B. bifidum 450B and may be due to the less amount of carbohydrates and the high content of non-soluble fiber
that affected the nutrients availability for the bacterial strains. The pH changes indicated the presence of shortchain fatty acids (SCFAs), being acetate (46–100%) the main SCFA. Changes in the proteome concerned proteins
that are involved in carbohydrate and other carbohydrate pathways. The characterization of the bacteria according to the growth, metabolites, and proteins expressed allows understanding the response to the change of environmental conditions and could be useful to understand L. acidophilus LA-5 and B. bifidum 450B strains’ adaptation to specific applications.

16 ART 2017 copia


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